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jackson immunoresearch 111 205 144  (Jackson Immuno)


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    Jackson Immuno jackson immunoresearch 111 205 144
    Jackson Immunoresearch 111 205 144, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jackson+immunoresearch+111+205+144/Colloidal+Gold+AffiniPure+Goat+Anti-Rabbit+IgG/pm41482220-84-35-35
    Average 94 stars, based on 273 article reviews
    jackson immunoresearch 111 205 144 - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands.
    Article Snippet: Sections were first blocked with 4% BSA + 2% whole goat serum (Fisher Scientific, # NBO36768) + 0.1% Tween 20 (Fisher Scientific, #MP1TWEEN201) in PBS for 30 min. After blocking, sections were incubated on drops of the primary antibody (polyclonal anti-DsRed, Clontech Ab # 632496), diluted 1:200 in 2% BSA + 1% whole goat serum + 0.1% Tween 20 in PBS overnight at 4 C. Following incubation in primary antibody, the sections were washed twice (5 min each) in PBS. .. The sections were then incubated in secondary antibody (12 nm gold-conjugated goat anti-rabbit IgG (H+L), Jackson ImmunoResearch, # 111-205-144) diluted 1:20 in PBS at room temperature for 2 h. Sections were then washed with PBS and post-fixed with 2% glutaraldehyde in water for 5 min. .. Following post-fixation, the sections were washed twice (2 min each) with water, stained with uranyl acetate (Electron Microscopy Sciences, #22400) for 2 min and then washed briefly with water.

    Labeling:

    Article Title: The plastidial phosphorylase Pho1 co-localizes in situ with Photosystem I-associated PsaC as assessed by double labeling immunocytochemistry - transmission electron microscopy.
    Article Snippet: Ultrathin sections (90–100 nm) were blocked with 10 % BSA in TBS (50 mM Tris, 150 mM NaCl, pH 7.5) for 30 min, incubated with both a laboratory generated anti-Pho1 antibody (rabbit; 1:200) and a monoclonal anti-GFP antibody (mouse; Rockland, 800–656–7625) in 1 % BSA for 2 h (1:50 dilution) or overnight (1:100 dilution). .. After washing with TBS (5 times for 2 min each for 1:50 dilution while the 1:00 dilution was washed once for 5 min), sections were labeled with goat anti-rabbit IgG conjugated with 12 nm gold (Jackson ImmunoResearch, 111–205–144) and goat anti-mouse IgG conjugated with 6 nm gold (Jackson ImmunoResearch, 115–195–166) at a dilution of 1:20 in 1 % BSA for 30 min, and then stained with 2 % uranyl acetate for 8 min and lead citrate for 8 min. ..

    Staining:

    Article Title: The plastidial phosphorylase Pho1 co-localizes in situ with Photosystem I-associated PsaC as assessed by double labeling immunocytochemistry - transmission electron microscopy.
    Article Snippet: Ultrathin sections (90–100 nm) were blocked with 10 % BSA in TBS (50 mM Tris, 150 mM NaCl, pH 7.5) for 30 min, incubated with both a laboratory generated anti-Pho1 antibody (rabbit; 1:200) and a monoclonal anti-GFP antibody (mouse; Rockland, 800–656–7625) in 1 % BSA for 2 h (1:50 dilution) or overnight (1:100 dilution). .. After washing with TBS (5 times for 2 min each for 1:50 dilution while the 1:00 dilution was washed once for 5 min), sections were labeled with goat anti-rabbit IgG conjugated with 12 nm gold (Jackson ImmunoResearch, 111–205–144) and goat anti-mouse IgG conjugated with 6 nm gold (Jackson ImmunoResearch, 115–195–166) at a dilution of 1:20 in 1 % BSA for 30 min, and then stained with 2 % uranyl acetate for 8 min and lead citrate for 8 min. ..



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